Actinomycete nucleic acid extraction detection reagent, kit and method and application thereof

The invention provides an actinomycete nucleic acid extraction and detection reagent, a kit and a method and application thereof, the extraction and detection reagent contains InstaGeneMatrix, Tris-HCl, SDS, KCl and EDTA, the extraction and detection reagent can rapidly and efficiently extract mycobacterium tuberculosis nucleic acid DNA, and the extraction and detection limit of mycobacterium tuberculosis is increased to 8 copy number/ml; mycobacterium tuberculosis nucleic acid can be rapidly and efficiently extracted from a complex sample, and the potential clinical applicability of the extraction method is verified. 本发明提供了一种放线菌核酸提取检测试剂、试剂盒及其方法和应用，所述提取检测试剂含有InstaGeneMatrix、Tris-HCl、SDS、KCl和EDTA，本发明能够实现快速高效提取结核分枝杆菌核酸DNA，将结核分枝杆菌的提取检测限提高到8拷贝数/ml；从复杂样本中亦能快速高效提取到结核分枝杆菌核酸，验证了本提取方法潜在的临床适用性。