Construction and application of bovine CART gene eukaryotic overexpression vector

The invention discloses construction and application of a cattle CART gene eukaryotic overexpression vector. The construction specifically comprises the following steps: step 1, obtaining a cattle CART mRNA + UTR sequence fragment by using a PCR method; step 2, cloning a target gene CART mRNA + UTR into a vector pEX-3; step 3, overexpression of CART mRNA + UTR in the 293T cell: culturing the 293T cell; and the pEX-3-CART mRNA + UTR recombinant overexpression vector is used for transfecting a 293T cell. The method has the technical effects that the regulation of miRNA in primary cells on an mRNA UTR (Untranslated Region) region can be better simulated; a foundation is laid for researching a CART posttranscriptional regulation mechanism and a CART regulation and control bovine follicle development mechanism, and the overexpression condition of pEX-3-CART mRNA + UTR is detected through expression of CART mRNA and CART protein. 本发明公开了一种牛CART基因真核过表达载体的构建与应用，具体包括以下步骤：步骤一：利用PCR方法得到牛CART mRNA+UTR序列片段；步骤二：目的基因CART mRN