Instantaneous transformation method for acanthopanax senticosus cells

The invention relates to a method for obtaining radix acanthopanacis senticosi callus single cells by utilizing an enzymolysis method and carrying out agrobacterium transformation on the radix acanthopanacis senticosi callus single cells to obtain GFP (Green Fluorescent Protein) transgene, belonging to the technical field of biology. When 1.5% cellulase R-10 and 0.2% macerozyme R-10 are adopted for enzymolysis of the acanthopanax callus for 6 h and then 400 * g centrifugation is conducted for 5 min, the single cell yield is the maximum, the activity is the strongest, and the single cells are 1.88 * 10 < 6 > * g and 90% respectively. When the agrobacterium tumefaciens transforms the single cell for 40 minutes under the PEG concentration of 40%, the instantaneous transformation efficiency of the single cell reaches 30.34%. The invention provides an enzymolysis system for high-yield acanthopanax senticosus single cells, simultaneously obtains consistent single cells of transgenic GFP (Green Fluorescent Pro