Eucommia staminate flower and eucommia staminate flower extract HPLC characteristic chromatogram construction method

The invention discloses an establishment method of an HPLC (High Performance Liquid Chromatography) specific chromatogram of eucommia male flowers and extracts thereof and a fingerprint spectrum of the eucommia male flowers and the extracts thereof. The method comprises the following steps: step 1, preparing a test solution; 2, preparing a reference substance solution; step 3, chromatographic conditions: octadecylsilane chemically bonded silica is used as a filling agent; a mobile phase A is acetonitrile, a mobile phase B is a 0.1% phosphoric acid solution, and gradient elution is carried out; the detection wavelength is 206 nm; the column temperature is 10-30 DEG C; the flow rate is 1.0 ml.min ; the sample size is 10 microliters; and 4, determining to obtain the fingerprint spectrum of the eucommia male flowers. The invention also performs methodological verification on the establishment method of the fingerprint spectrum. The characteristic chromatogram of the eucommia male flower and the eucommia male