DNA methylation photoelectric detection method for TiO2 defect regulation and control
The invention provides a TiO2 defect-regulated DNA methylation photoelectric detection method which comprises the following steps: S1, taking a ZnO nanorod array electrode as a template, and carrying out liquid phase deposition for multiple times in a precursor solution containing zinc and titanium...
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| creator | ZHUANG HUANGLONG XU PINSHENG CHEN FENG LIN LINGLING YE CHENQING |
| description | The invention provides a TiO2 defect-regulated DNA methylation photoelectric detection method which comprises the following steps: S1, taking a ZnO nanorod array electrode as a template, and carrying out liquid phase deposition for multiple times in a precursor solution containing zinc and titanium at the same time to obtain a zinc-doped TiO2-x nanotube array electrode with controllable surface oxygen vacancy concentration; s2, taking EDAC as a coupling agent and benzotriazole as a reaction intermediate, enabling dihydroxybenzoic acid to react with the capture probe ssDNA of which one end is modified with amino to form an amido bond to obtain ssDNA-PCA molecules; s3, the TiO2-x nanotube array electrode is immersed in a solution of ssDNA-PCA molecules, an assembly reaction is carried out at the room temperature, and an ssDNA-PCA/TiO2-x nanotube array electrode is formed; s4, carrying out denaturation on the DNA methylation sample, and adding sodium hydrogen sulfite to carry out pretreatment on the DNA methylat |
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s2, taking EDAC as a coupling agent and benzotriazole as a reaction intermediate, enabling dihydroxybenzoic acid to react with the capture probe ssDNA of which one end is modified with amino to form an amido bond to obtain ssDNA-PCA molecules; s3, the TiO2-x nanotube array electrode is immersed in a solution of ssDNA-PCA molecules, an assembly reaction is carried out at the room temperature, and an ssDNA-PCA/TiO2-x nanotube array electrode is formed; s4, carrying out denaturation on the DNA methylation sample, and adding sodium hydrogen sulfite to carry out pretreatment on the DNA methylat</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; 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s2, taking EDAC as a coupling agent and benzotriazole as a reaction intermediate, enabling dihydroxybenzoic acid to react with the capture probe ssDNA of which one end is modified with amino to form an amido bond to obtain ssDNA-PCA molecules; s3, the TiO2-x nanotube array electrode is immersed in a solution of ssDNA-PCA molecules, an assembly reaction is carried out at the room temperature, and an ssDNA-PCA/TiO2-x nanotube array electrode is formed; s4, carrying out denaturation on the DNA methylation sample, and adding sodium hydrogen sulfite to carry out pretreatment on the DNA methylat</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE |
| title | DNA methylation photoelectric detection method for TiO2 defect regulation and control |
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