DNA methylation photoelectric detection method for TiO2 defect regulation and control

The invention provides a TiO2 defect-regulated DNA methylation photoelectric detection method which comprises the following steps: S1, taking a ZnO nanorod array electrode as a template, and carrying out liquid phase deposition for multiple times in a precursor solution containing zinc and titanium at the same time to obtain a zinc-doped TiO2-x nanotube array electrode with controllable surface oxygen vacancy concentration; s2, taking EDAC as a coupling agent and benzotriazole as a reaction intermediate, enabling dihydroxybenzoic acid to react with the capture probe ssDNA of which one end is modified with amino to form an amido bond to obtain ssDNA-PCA molecules; s3, the TiO2-x nanotube array electrode is immersed in a solution of ssDNA-PCA molecules, an assembly reaction is carried out at the room temperature, and an ssDNA-PCA/TiO2-x nanotube array electrode is formed; s4, carrying out denaturation on the DNA methylation sample, and adding sodium hydrogen sulfite to carry out pretreatment on the DNA methylat