Ambroxol hapten, artificial antigen, antibody and preparation method and application thereof

The invention belongs to the technical field of food safety detection, and particularly provides an ambroxol hapten, an artificial antigen, an antibody and a preparation method and application thereof.The ambroxol hapten Abx-SAA is prepared, the ambroxol hapten Abx-SAA is coupled with a carrier protein BSA to obtain the artificial antigen Abx-SAA-BSA, and the artificial antigen Abx-SAA-BSA is used for preparing the ambroxol hapten Abx-SAA-BSA. A specific antibody for detecting ambroxol is further prepared, and an artificial antigen Abx-SAA-OVA is used as a coating antigen; the antibody has good sensitivity and specificity to ambroxol, the semi-inhibitory concentration is 3.01 [mu] g/L, the lowest detection limit is 0.17 [mu] g/L, the quantitative detection range is 0.50-20.61 [mu] g/L, and no cross reaction is generated to bromhexine and brombuterol. By using the antibody, an ambroxol immunoassay method with strong specificity and high sensitivity is established. 本发明属于食品安全检测技术领域，具体提供了一种氨溴索半抗原、人工抗原、抗体及其制备方法和应用