Primer group, kit and sequencing library for detecting large fragment deletion of rare beta thalassemia gene with unclear fracture site

The invention relates to the field of gene detection, in particular to a primer group, a kit and a sequencing library for detecting large fragment deletion of a rare beta thalassemia gene with an unclear fracture site. A pair of complementary primers which are far away from each other are designed in upstream and downstream regions of a beta-globin gene cluster, a pair of normal internal control complementary primers which are moderate in distance is designed in an HBB gene region, long-fragment PCR amplification is performed, and a third-generation sequencing library is constructed and sequenced; the accurate and rapid detection of the large-fragment deletion variation of the rare beta thalassemia gene with the unclear fracture site is realized. Besides, according to the method, DNA of different sequences of 5-50 nt can be added to the 5'end of the designed primer, namely a DNA barcode (Barcode) for distinguishing different samples and achieving simultaneous detection of multiple samples, and the method is e