Bst DNA polymerase mutant as well as application, product, gene, recombinant plasmid and genetically engineered bacterium thereof

The invention provides a Bst DNA polymerase mutant as well as application, a product, a gene, a recombinant plasmid and a genetically engineered bacterium thereof, and relates to the technical field of biology. According to the Bst DNA polymerase mutant provided by the invention, Met at the 370th site of a wild type Bst DNA polymerase is mutated into Gly, and Ala at the 444th site of the wild type Bst DNA polymerase is mutated into Gly. The Bst DNA polymerase mutant has the advantages of good thermal stability, reduced non-specific amplification reaction and good repeatability, and can be applied to the loop-mediated isothermal amplification technology. 本发明提供了一种Bst DNA聚合酶突变体及其应用和产品、基因、重组质粒和基因工程菌，涉及生物技术领域。本发明提供的Bst DNA聚合酶突变体，将野生型Bst DNA聚合酶的第370位的Met突变为Gly，第444位的Ala突变为Gly。该Bst DNA聚合酶突变体热稳定性好，非特异性扩增反应减少，重复性好，能够应用于环介导等温扩增技术中。