Preparation method of marine oligosaccharides with antitumor activity, marine oligosaccharides and application of marine oligosaccharides

The invention discloses a preparation method of marine oligosaccharides with anti-tumor activity, marine oligosaccharides and application of the marine oligosaccharides, the preparation method comprises the following steps: S1, uniformly mixing chitosan with an organic acid solution to prepare a chitosan solution; s2, carrying out degradation by adopting ultrahigh pressure under the degradation conditions that the temperature is 45-55 DEG C, the pressure is 250-400 MPa, and the pressure holding time is 15-30 minutes, so as to obtain a chitosan degradation solution; s3, the chitosan degradation solution obtained in the step S2 is centrifuged, concentrated and dried, and the marine oligosaccharides with the high anti-tumor activity are obtained, wherein the molecular weight of the marine oligosaccharides is 800-1200 Da, and the proportion of the marine oligosaccharides is 60% or above. The preparation method of the marine oligosaccharides with the anti-tumor activity is simple, short in time, low in temperature