Kit as well as preparation method and application thereof

The invention discloses a kit and a preparation method and application thereof, and relates to the technical field of immunodetection analysis, the kit is used for detecting the content of soluble fms-like tyrosine kinase-1, the kit comprises: a first reagent comprising streptavidin magnetic beads and a first diluent; the second reagent comprises an enzyme-labeled soluble fms-like tyrosine kinase-1 monoclonal antibody and a second diluent; the third reagent comprises NHS-LC-biotin marked with a soluble fms-like tyrosine kinase-1 monoclonal antibody and a third diluent; a calibrator; and a light-emitting substrate. When the kit provided by the invention is used for detecting the content of soluble fms-like tyrosine kinase-1, the sensitivity is high, the stability and the repeatability are good, and the anti-interference capability is strong. 本发明公开一种试剂盒及其制备方法和应用，涉及免疫检测分析技术领域，所述试剂盒用于检测可溶性fms样酪氨酸激酶-1的含量，所述试剂盒包括：第一试剂，包括链霉亲和素磁珠和第一稀释液；第二试剂，包括酶标记的可溶性fms样酪氨酸激酶-1单克隆抗体和第二稀释液；第三试剂，包括标记可溶性fms样酪氨酸激酶-1单克隆抗体的NHS-LC-生物素和第三稀释液