Gold nanoparticle film SALDI-MS substrate fixed on sulfydryl-modified silicon substrate and application of gold nanoparticle film SALDI-MS substrate in detection of small molecules

The invention discloses a gold nanoparticle film SALDI-MS substrate fixed on a sulfydryl modified silicon substrate and application of the gold nanoparticle film SALDI-MS substrate in detection of small molecules, and belongs to the technical field of mass spectrometry. The substrate is obtained by transferring an assembled gold nanoparticle film to a silicon substrate with the surface modified by sulfydryl, the process is simple, safe and repeatable, and special equipment or instruments are not needed. According to the method, after being uniformly self-assembled, the gold nanoparticles are transferred to the silicon substrate of which the surface is modified with sulfydryl functional groups, so that the binding force between the gold nanoparticles and the substrate is enhanced, and the influence on mass spectrometric detection due to separation of a gold nanoparticle film is avoided; the gold nanoparticles are uniformly distributed to form a film, so that the formation of coffee rings caused by non-uniform