Primers, probes and method for typing detection of five types of input plasmodium
The invention discloses a primer, a probe and a method for typing detection of five types of input plasmodium. The five types of input plasmodium comprise plasmodium falciparum, plasmodium vivax, plasmodium malvax, plasmodium ovale and plasmodium noiri, the number of the primers is eight, and the nu...
Gespeichert in:
Hauptverfasser: | , , , , , , |
---|---|
Format: | Patent |
Sprache: | chi ; eng |
Schlagworte: | |
Online-Zugang: | Volltext bestellen |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The invention discloses a primer, a probe and a method for typing detection of five types of input plasmodium. The five types of input plasmodium comprise plasmodium falciparum, plasmodium vivax, plasmodium malvax, plasmodium ovale and plasmodium noiri, the number of the primers is eight, and the number of the probes is two. On the basis of multiple PCR, a large number of long-chain and single-chain DNA products with specific tag sequences are amplified through an asymmetric amplification method, and rapid detection and typing of five kinds of plasmodium with single tube/closed tube double channels are achieved in combination with a universal two-dimensional labeled probe mediated melting curve analysis technology. Meanwhile, whether the system is normal or not and the sample collection quality can be indicated through an internal label. The lower detection limit of five detection objects of the detection system is 10 copy number/reaction, and the system is good in specificity and high in selectivity. The met |
---|