Targeted DNA demethylation method, fusion protein and application thereof

The invention relates to a targeted DNA (Deoxyribose Nucleic Acid) demethylation method (Mini-CRISD) and a fusion protein used in the method. According to the Mini-CRISD method disclosed by the invention, the engineered narrowed ROS1 demethylation effector is delivered to a specific DNA sequence and...

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Bibliographische Detailangaben
Hauptverfasser:	ZHU YUGUANG, SHEN AO, ZHANG LINGMIN, YU XIYONG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a targeted DNA (Deoxyribose Nucleic Acid) demethylation method (Mini-CRISD) and a fusion protein used in the method. According to the Mini-CRISD method disclosed by the invention, the engineered narrowed ROS1 demethylation effector is delivered to a specific DNA sequence and/or a specific genome position (such as a CpG island) through the engineered narrowed dCjCas9 to deliver the demethylation activity in a targeted manner, and the targeted demethylation of specific DNA can be realized. 本发明涉及一种靶向的DNA去甲基化方法(Mini-CRISD)和该方法中所用到的融合蛋白。本发明所述Mini-CRISD方法通过工程化的缩小dCjCas9递送工程化的缩小ROS1去甲基化效应子至特定DNA序列和/或特定基因组位置(如CpG岛)来靶向递送去甲基化活性，可以对特定DNA实现靶向去甲基化。