Rapid visual spermidine detection method based on in-situ pre-modification technology

The invention relates to the field of spermidine detection, and discloses a rapid visual spermidine detection method based on an in-situ pre-modification technology, spermidine is pre-modified by o-phthalaldehyde, and a perylene bisimide derivative with a structure as shown in a formula (I) is used as a probe. The perylene bisimide derivative has good water solubility, and spermidine can be visually detected with high sensitivity and specificity. The detection method provided by the invention has relatively high detection efficiency and sensitivity; 本发明涉及亚精胺检测领域，公开了一种基于原位预修饰技术的亚精胺快速可视化检测方法，亚精胺经邻苯二甲醛进行预修饰，以式(I)所示结构的苝酰亚胺衍生物作为探针。所述苝酰亚胺衍生物具有良好的水溶性，并且能够实现高灵敏度、特异性且可视化地检测亚精胺。本发明提供的检测方法具有较高的检测效率和灵敏度；