Preparation method and application of AgNPs (at) SASP substrate material

The invention discloses a preparation method and application of an AgNPs (at) SASP substrate material, cyclohexane is taken as an organic phase, ethanol is taken as an inducer, and self-assembly is carried out by utilizing interfacial tension of an organic/water interface, so that nanoparticles are arranged in a single layer. A low-dielectric solvent is added to serve as an inducer to reduce the charge density result on the nanoparticles, Van der Waals force and coulomb repulsive force are reduced, so that the nanoparticles have the tendency of reassembling on an organic/water interface, a new balance is formed, and an SERS platform of a two-dimensional AgNPs array is formed. Different food-borne pathogenic bacteria spores are subjected to SERS (Surface Enhanced Raman Scattering) detection and peak position attribution analysis, and qualitative analysis is performed through a multivariate statistics method, so that rapid identification of spore bacteria is realized. 本发明公开了一种AgNPs@SASP基底材料的制备方法及应用，以环己烷为有机相以乙醇为