Method for improving yield of cell factors of stem cells

The invention belongs to the technical field of biology, and discloses a method for improving the yield of cell factors of stem cells, which comprises the following steps: inoculating adipose-derived stem cells into a T25 culture flask containing a culture medium for culturing; when the fusion degree of the stem cells reaches 75-85%, irradiating the cell culture solution with a xenon lamp, culturing again, and collecting a stem cell factor supernatant; filtering the stem cell factor supernate by using a filter membrane, and sequentially passing through a 50KD ultrafiltration membrane and a 1KD ultrafiltration membrane to obtain a cell factor concentrated solution; and adding a freeze-drying protective agent into the cell factor concentrated solution, and carrying out freeze-drying treatment to obtain the freeze-dried powder containing the stem cell factors with increased content. The method for increasing the yield of the stem cell factors is simple and efficient, and the total yield of the stem cell factors