Application of strawberry MYB transcription factor FaMYB5 gene

The invention relates to the technical field of plant molecular genetic engineering, in particular to application of a strawberry MYB transcription factor FaMYB5 gene, by cloning the strawberry MYB transcription factor FaMYB5 gene and an expression vector and host bacteria containing the FaMYB5 gene...

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Bibliographische Detailangaben
Hauptverfasser: JIANG RONGYI, LIU YAXIN, PAN ZHIFEI, ZHAO JING, FANG CONGBING, SUN PEIPEI, XIE XINGBIN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to the technical field of plant molecular genetic engineering, in particular to application of a strawberry MYB transcription factor FaMYB5 gene, by cloning the strawberry MYB transcription factor FaMYB5 gene and an expression vector and host bacteria containing the FaMYB5 gene, the FaMYB5 gene can be applied to regulation and control of citric acid accumulation in strawberry fruits and expression of CS1 and GAD genes, and the strawberry MYB transcription factor FaMYB5 gene is obtained. The FaMYB5 is overexpressed in strawberries to promote citric acid metabolism of strawberry fruits, meanwhile, the expression quantity of a main gene CS1 for citric acid synthesis is increased, and the expression quantity of a degradation gene GAD is obviously reduced. 本发明涉及植物分子基因工程技术领域,具体涉及一种草莓MYB转录因子FaMYB5基因的应用,通过克隆出草莓MYB转录因子FaMYB5基因以及含有上述FaMYB5基因的表达载体、宿主菌,这种FaMYB5基因能够应用于调控草莓果实中柠檬酸积累、表达CS1和GAD基因,在草莓中过量表达FaMYB5促进草莓果实柠檬酸代谢,同时柠檬酸合成主要基因CS1表达量上升和降解基因GAD表达量明显下降。