Method for detecting whole-spectrum vitamins in serum

The invention discloses a method for detecting whole-spectrum vitamins in serum, and relates to the technical field of metabolome analys.The method comprises the steps that isotope vitamins are added into a sample to serve as an internal standard, then water-soluble vitamins and fat-soluble vitamins in the sample are extracted through a solid-liquid extraction method, and after the vitamins are separated through a T3 chromatographic column, the total-spectrum vitamins in the sample are obtained; and two-time sample injection and one-time analysis are carried out in a segmented manner. Compared with the prior art, the method not only solves the difficulty that the whole spectrum of vitamins cannot be completed at one time, but also greatly reduces the sample size, and has the advantages of high efficiency, high accuracy, short analysis time and the like. 本发明公开了一种血清中全谱维生素的检测方法，涉及代谢组分析技术领域，其先向样本中加入同位素维生素作为内标，再通过固-液萃取法分别提取样本中的水溶性维生素和脂溶性维生素，使用T3色谱柱将维生素分离后，采用分段两次进样一次分析。相比于现有技术，不仅解决了无法一次完成全谱维生素的困难，而且大大降低了样本量，并具有效率高、