Method for simultaneously determining multiple water-soluble vitamins

The invention relates to the field of vitamin detection and analysis, in particular to a method for simultaneously determining multiple water-soluble vitamins. According to the method, high performance liquid chromatography is adopted for detection and analysis, a test solution is prepared by adding a to-be-detected sample into an acidified ethanol solution, performing ultrasonic treatment, filtering and adding a solvent for dissolving, and the acidified ethanol is hydrochloric acid ethanol or glacial acetic acid ethanol. The method can avoid interference of various components in a sample containing multiple nutritional components such as calcium, magnesium, iron, zinc and copper, is simple, convenient and safe, only needs one-time sample pretreatment, adopts a mobile phase, is simple in reagent, and can simultaneously determine the content of five vitamins through one-time sample feeding. 本发明涉及维生素检测分析领域，具体涉及一种同时测定多种水溶性维生素的方法。所述方法采用高效液相色谱进行检测分析，其中供试品溶液的制备包括将待测样品加入酸化乙醇溶液中，超声，过滤，加溶剂溶解即得，所述酸化乙醇为盐酸乙醇或冰乙酸乙醇，可以避免含有