Method for separating free-state and lipidosome-state esculentin in biological matrix

The invention discloses a method for separating free-state and lipidosome-state esculentin in a biological matrix, which comprises the following steps: transferring the free-state and lipidosome-state esculentin in the biological matrix to a solid-phase extraction column, and leaching and eluting to separate the free-state and lipidosome-state esculentin in the biological matrix. According to the present invention, based on the selection of the solid phase extraction column with the appropriate filler, the optimal elution reagent and the optimal elution reagent, the free esculentin and the liposome esculentin in the biological matrix can be separated, and the quantitative detection of the free esculentin and the total esculentin in the biological matrix can be finally supported. By using the method, the recovery rate of the free-state lipidosome can reach 90% or above, and the requirement of quantitative detection is basically met. 本发明公开了一种分离生物基质中游离态和脂质体态艾立布林的方法，将生物基质中游离态和脂质体态艾立布林转移至固相萃取柱，经淋洗、洗脱后，使生物基质中游离态和脂质