Molecular marker of rice gelatinization temperature gene ALK as well as primer and application of molecular marker

The invention discloses a molecular marker of a rice gelatinization temperature gene ALK as well as a primer and application of the molecular marker. Nucleotide sequences of the molecular marker are shown as SEQ ID NO.3 and SEQ ID NO.4. The nucleotide sequence of the high-alkali elimination rice amp...

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Bibliographische Detailangaben
Hauptverfasser: LIU LIPING, LIU SONG, LYU JIANQUN, REN JUANSHENG, GAO FANGYUAN, REN GUANGJUN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a molecular marker of a rice gelatinization temperature gene ALK as well as a primer and application of the molecular marker. Nucleotide sequences of the molecular marker are shown as SEQ ID NO.3 and SEQ ID NO.4. The nucleotide sequence of the high-alkali elimination rice amplification product is as shown in SEQ ID No.3, the nucleotide sequence of the medium-alkali elimination rice amplification product is as shown in SEQ ID No.4, and the heterozygous genotype rice material is simultaneously amplified into bands as shown in SEQ ID No.3 and SEQ ID No.4. According to the invention, the identification of the rice molecular marker assisted breeding high/medium alkali elimination value material can be rapidly detected by using a simple and reliable method. 本发明公开了一种水稻糊化温度基因ALK的分子标记及其引物和应用,该分子标记物的核苷酸序列如SEQ ID NO.3和SEQ ID NO.4所示。高碱消值水稻扩增产物的核苷酸序列如SEQ ID No.3所示,中碱消值水稻扩增产物的核苷酸序列如SEQ ID No.4所示,杂合基因型水稻材料同时扩增出如SEQ ID No.3所示和如SEQ ID No.4所示的条带。本发明能够实现使用简单可靠的方法快速检测水稻分子标记辅助育种高/中碱消值材料的鉴定。