Primer set for mitochondrial whole genome sequencing and high-throughput sequencing method

The invention discloses a primer set for mitochondrial whole genome sequencing and a high-throughput sequencing method, and relates to the technical field of genome sequencing. The primer set comprises at least two primer subsets, and the primer set comprises 101 pairs of primers as shown in SEQ ID NO. 1-202. According to the primer set and the high-throughput sequencing method, the operation steps are simplified to a great extent, higher sequencing depth can be obtained under the condition of less data volume, and the sequencing cost is reduced. And even for a low-quality FFPE sample, the high-throughput sequencing requirement also can be met. 本发明公开了一种线粒体全基因组测序的引物集及高通量测序的方法，涉及基因组测序技术领域。引物集包括：至少两个引物子集，引物集包括如SEQ ID NO.1-202所示的101对引物。该引物集及高通量测序方法极大程度上精简了操作步骤，满足较少数据量条件下也能获得更高的测序深度，降低测序成本。甚至对于低质量的FFPE样品也能满足高通量测序需求。