Method for increasing proportion of 4-hydroxybutyric acid monomer in polyhydroxyalkanoate

The invention belongs to the technical field of biopolymer materials, and discloses a method for increasing the content of 4-hydroxybutyric acid in polyhydroxyalkanoate. The method comprises the following steps: adding a monomer regulator into a fermentation system to prepare P (3HB-co-4HB); the monomer regulator is selected from at least one of propionate and butyrate. By adopting the method, the proportion of the monomer in the product P (3HB-co-4HB) can be adjusted, and the effect of increasing the content of the 4-hydroxybutyric acid monomer in the P (3HB-co-4HB) is achieved. In addition, propionate and butyrate are used as alkaline strong alkali and weak acid salts, so that the use amount of alkaline pH regulators such as sodium hydroxide in the fermentation process can be effectively reduced, and the cost is saved. 本发明属于生物高分子材料技术领域，公开了一种提高聚羟基脂肪酸酯中4-羟基丁酸含量的方法。该方法通过在发酵体系中加入单体调节剂，制得P(3HB-co-4HB)；所述单体调节剂选自丙酸盐、丁酸盐中的至少一种。采用该方法可调节产物P(3HB-co-4HB)中的单体比例，起到提高P(3HB-co-4HB)中4-羟基丁酸单体含量的作用。此外，丙酸盐、丁酸盐作为呈碱性的强碱弱酸盐，可以有效减