具有亚砷酸盐和甲基亚砷酸盐结合能力的蛋白A9、含所述蛋白基因的工程菌株及应用

本发明提供砷结合蛋白A9、含有该砷结合蛋白A9的编码基因的重组载体和含有上述重组载体的重组工程菌株。本发明的砷结合蛋白A9可提高菌株对亚砷酸盐和甲基亚砷酸盐抗性的应用，提高菌株对亚砷酸盐和甲基亚砷酸盐结合率。本发明的砷结合蛋白A9实现在体外结合亚砷酸盐和甲基亚砷酸盐。本发明的重组工程菌对亚砷酸盐(60μM)和甲基亚砷酸盐(3μM)具有很强的抗性，对亚砷酸盐和甲基亚砷酸盐吸收效率高达89.71％和84.95％、目的蛋白A9对亚砷酸盐和甲基亚砷酸盐吸收效率高达89.08％和88.27％，能够有效去除培养环境中砷。本发明的砷结合蛋白为南方水稻田和水体环境的砷污染修复提供了一种新的生物修复技术。 The invention provides an arsenic binding protein A9, a recombinant vector containing a coding gene of the arsenic binding protein A9 and a recombinant engineering strain containing the recombinant vector. The arsenic binding protein A9 disclosed by the invention can be applied to improving the resistance of a strain to arsenite and methyl arsenite, and the binding rate of the strain to arsenite and methyl arsenite is improved. The arsenic binding protein A9 disclosed by the invention realizes in-vitro binding of arsenite and methyl arsenite. The recombinant engineering bacterium has very strong resistance to arsenite (60 [mu] M) and methyl arsenite (3 [mu] M), the absorption efficiency of the target protein A9 to th