Preparation of sulfated polysaccharide-folic acid conjugate synthesized nanoparticles

The preparation method of the sulphated polysaccharide-folic acid conjugate synthesized nano-particles comprises the following steps: dissolving deoxycholic acid in distilled water, respectively adding 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide and N-hydroxysuccinimide which are 1.2 times of the equivalent weight of deoxycholic acid, enabling the 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide and the N-hydroxysuccinimide to react with amino groups in sulphated polysaccharide to form amido bonds, and preparing the sulphated polysaccharide-folic acid conjugate synthesized nano-particles. The preparation method comprises the following steps: adding activated deoxycholic acid into a sulfated polysaccharide-folic acid conjugate solution, fully mixing, precipitating in a phosphate buffer solution to prepare a sulfated polysaccharide-folic acid compound, dialyzing the reacted compound in water, freeze-drying, dissolving the modified sulfated polysaccharide-folic acid compound in the phosphate buffer solution, slow