Lentivirus packaging method

The invention provides a lentivirus packaging method which comprises the following steps: (1) adding plasmids into a serum-free culture medium to obtain a plasmid mixture; the plasmids comprise lentivirus packaging plasmids and target expression plasmids; the lentivirus packaging plasmids comprise psPAX2 and pMD2. G; (2) adding the transfection medium into a serum-free culture medium to obtain a transfection reagent; and (3) uniformly mixing the plasmid mixture and a transfection reagent, adding the mixture into the tool cells, transfecting and culturing for 2-10 hours, replacing the culture medium with a serum-reduced culture medium, continuously culturing, collecting a supernatant, and concentrating to obtain the lentivirus. In the lentivirus packaging method provided by the invention, the target expression plasmid and the two packaging plasmids are utilized to carry out co-transfection on the tool cells, so that the lentivirus can stably package the tool cells, and the lentivirus packaging efficiency and t