Red fluorescence labeled cell with human retina differentiation potential and construction method thereof

The invention relates to a red fluorescence labeled cell with human retina differentiation potential and a construction method thereof. The construction method comprises the step of inserting a red fluorescent protein expression cassette into a human stem cell genome. According to the invention, a red fluorescence labeled embryonic stem cell line is constructed by designing a targeting vector and related molecular genetic manipulation, and the cell line can be induced and differentiated into a retinal organ expressing tdTomato red fluorescence through 3D culture. The obtained retina-like organ is consistent with nerve cells of a normal human retina in composition, and the development time and space sequence of the obtained retina-like organ are close to those of the normal human retina. The cell line is a powerful tool, can help to realize related research on human retina development and disease generation, and promotes development of a blinding disease treatment method. 本发明涉及一种具有人视网膜分化潜能的红色荧光标记细胞及其构建方法，包括将红色