Purification process of mussel-like mucin

The invention discloses a purification process of mussel-like mucin, and belongs to the field of protein purification. The purification process comprises the following steps: (1) breaking cells; (2) acid extraction; (3) adding salt to remove impurities, namely adding salt into the concentrated solution obtained in the step (2) until the final concentration is 0.20-0.80 M, centrifuging, collecting supernate, adding salt into the supernate until the final concentration is 1-3 M, centrifuging, and collecting precipitate; (4) enzyme catalytic oxidation; and (5) ion exchange chromatography. The mussel-like mucin with high yield, high purity and high dopa content is obtained by using the purification process provided by the invention. The purification process disclosed by the invention is short in steps and low in cost, and has a wide application prospect in large-scale production of the mussel-like mucin. 本发明公开了一种类贻贝粘蛋白的纯化工艺，属于蛋白纯化领域。该纯化工艺包括以下步骤：(1)破胞；(2)酸提；(3)加盐除杂：在步骤(2)的浓缩液中加入盐至终浓度为0.20～0.80M，离心，收集上清液，在上清液中加入盐至终