Cetilistat hapten, artificial antigen, antibody and preparation method and application thereof

The invention belongs to the technical field of food detection, and particularly provides a cetilistat hapten, an artificial antigen and an antibody as well as a preparation method and application of the cetilistat hapten, the artificial antigen and the antibody, two kinds of cetilistat hapten, hapten CET1 and hapten CET2 are prepared, the artificial antigen CET1 is obtained by applying the hapten CET1, and the specific antibody for detecting cetilistat is further prepared. According to the present invention, the artificial antigen CET2-OVA is adopted as the coating antigen, the lowest detection limit of the antibody on the Cetilistat is 0.22 ng/mL, the half inhibition concentration is 6.57 ng/mL, the antibody has good sensitivity and good specificity, the antibody is adopted to establish the Cetilistat immunoassay method, and the rapid and accurate detection of the Cetilistat is achieved. 本发明属于食品检测技术领域，具体提供了一种新利司他半抗原、人工抗原、抗体及其制备方法和应用，本发明制备得到了两种新利司他半抗原，半抗原CET1和半抗原CET2，应用半抗原CET1得到人工抗原CET1，并进一步制备得到用于检测新利司他的特异性抗