Dual fluorescent quantitative RT-PCR nucleic acid group, kit and method for synchronously detecting SGLV and YEZV

The invention relates to the field of virus detection, and discloses a dual fluorescent quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction) nucleic acid group for synchronously detecting SGLV and YEZV, a dual fluorescent quantitative RT-PCR kit for synchronously detecting SGLV and YEZV and a detection method in order to solve the problem of lack of a detection method for two tick-borne viruses, namely SGLV and YEZV, in the prior art. The method for synchronously detecting the SGLV and the YEZV is established, the detection speed is high, precious time is gained for treatment of patients, time and labor are saved when the two viruses are detected at a time, the reagent cost is low, the detection method is high in sensitivity, good in specificity and repeatable, interference of other viruses can be effectively eliminated, and the kit is convenient to use, easy to operate and suitable for popularization and application. The detection result is reliable. 本发明涉及病毒检测领域，为解决现有现有技术下缺少SGLV、YEZV两种蜱传病毒的检