Method for efficiently separating and purifying sugar and acid in bio-based material hydrolysate

The invention discloses a method for efficiently separating and purifying sugar and acid in bio-based material hydrolysate, which comprises the following steps of: loading the bio-based material hydrolysate into a reciprocating flow short bed which takes an amphoteric solid-phase separation material as a stationary phase, and recovering effluent in two sections, namely a dead volume area and a sugar recovery area; eluting from the other end of the reciprocating flow short bed by using an eluent, and collecting the recovered effluent in two sections, namely a sugar-acid mixed solution and an acid recovery area; repeating the steps until the solid-phase separation material has no separation effect; wherein the amphoteric solid-phase separation material takes a styrene-divinyl benzene copolymer as a skeleton structure, and the tail end of the styrene-divinyl benzene copolymer is a quaternary ammonium and carboxylic acid penetrating amphoteric group. 本发明公开了一种生物基材料水解液中的糖、酸高效分离纯化的方法，将生物基材料水解液上样到以两性固相分离材料为固定相的往复流动矮床