Cryopreservation preparation for antibody production cells, application of cryopreservation preparation and cryopreservation method

The invention discloses a cryopreservation preparation for antibody production cells, application of the cryopreservation preparation and a cryopreservation method. The cryopreserved preparation comprises 8 to 10% (v/v) of DMSO and 0.1 to 0.3% (w/v) of carboxymethyl cellulose and/or a salt thereof....

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Hauptverfasser: SHI WENLEI, SHEN YIJUN, YANG TONG, ZHAO TENG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a cryopreservation preparation for antibody production cells, application of the cryopreservation preparation and a cryopreservation method. The cryopreserved preparation comprises 8 to 10% (v/v) of DMSO and 0.1 to 0.3% (w/v) of carboxymethyl cellulose and/or a salt thereof. The cryopreservation preparation is simple in component, easy to implement and good in universality, and by means of the cryopreservation preparation and the cryopreservation method, the recovery motility rate of antibody production cells cryopreserved for a long time can be greatly increased. 本发明公开了一种抗体生产细胞的冻存制剂、其应用及冻存方法。所述冻存制剂包含8-10%(v/v)的DMSO、0.1-0.3%(w/v)的羧甲基纤维素和/或其盐。本发明所述冻存制剂的成分简单、易于实现,并具有较好的普适性,利用本发明所述的冻存制剂和冻存方法可以大幅提高长期冻存的抗体生产细胞的复苏活率。