Method for measuring squalene content

The invention provides a squalene content measurement method, which comprises: sample preparation: crushing a solid sample, and homogenizing a liquid sample; carrying out pretreatment on a sample to be detected; establishing a standard curve: selecting an internal reference compound to fix the content of the internal reference compound, changing the content of squalene, determining a standard curve by using a gas chromatograph, calculating the peak areas of squalene and the internal reference compound, dividing the peak areas by respective content ratios, and carrying out linear simulation to calculate a unit content peak area ratio k1; sequentially changing and fixing the content of an internal reference compound, changing the content of squalene, determining a standard curve, calculating unit content peak area ratios, and carrying out linear simulation to calculate unit content peak area ratios k2, k3, k4... kn; removing deviation values in each group of k values, and then taking an average value to obtain