Method for simultaneously determining vitamin A and derivatives thereof in cosmetics

The invention provides a method for simultaneously determining vitamin A and derivatives thereof in cosmetics. The method comprises the following steps: sampling; pre-treating and measuring the pre-treated sample solution; wherein the pretreatment step comprises the following steps: extraction: addi...

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Bibliographische Detailangaben
Hauptverfasser: LI GUAN, LIU YINGFANG, ZHANG JIAYUN, LAI LEI
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention provides a method for simultaneously determining vitamin A and derivatives thereof in cosmetics. The method comprises the following steps: sampling; pre-treating and measuring the pre-treated sample solution; wherein the pretreatment step comprises the following steps: extraction: adding a first specified amount of sodium chloride into a to-be-detected sample, adding a second specified amount of methanol solution, oscillating for specified time, and diluting with methanol to a third specified amount; purification: centrifuging, taking supernate, and filtering to obtain a pretreated sample solution; wherein the step of measuring the pretreated sample solution comprises the following steps: detection: detecting the pretreated sample solution by using HPLC-DAD (High Performance Liquid Chromatography-Diode Array Detection), and detecting the contents of the vitamin A and the derivative thereof in the pretreated sample solution according to a standard curve. The method can be used for simultaneously