Reagent and kit for detecting HPV16 and application

The invention belongs to the technical field of gene detection, and discloses a reagent for detecting HPV16, a kit and application. According to the reagent for detecting the HPV16, the HPV16 can be stably and sensitively detected by a one-tube one-step method, the gene copy number of a single digit...

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Bibliographische Detailangaben
Hauptverfasser:	HAO YU, LU QILAN, CHEN SONGBIN, QIU LIGONG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention belongs to the technical field of gene detection, and discloses a reagent for detecting HPV16, a kit and application. According to the reagent for detecting the HPV16, the HPV16 can be stably and sensitively detected by a one-tube one-step method, the gene copy number of a single digit can be detected, pipetting operation does not need to be carried out on an RT-RPA amplification product, so that the risk of aerosol pollution of the amplification product generated by the pipetting operation is eliminated, and the reagent is suitable for large-scale detection. 本发明属于基因检测技术领域，公开了检测HPV16的试剂、试剂盒及应用。该检测HPV16的试剂，可以实现同管一步法稳定、高灵敏地检测HPV16，可检测到个位数的基因拷贝数：无需对RT-RPA扩增产物实行移液操作，从而去除了移液操作产生的扩增产物气溶胶污染的风险，适合于规模化检测。