HSP/SAA recombinant protein as well as preparation method and application thereof

The invention discloses an HSP/SAA recombinant protein as well as a preparation method and application thereof. The HSP/SAA recombinant protein is formed by connecting an HSP gene and an SAA gene through a BglII/BamHI isocaudarner. Fusion proteins HSP and SAA are subjected to gene optimization and then are inserted into a prokaryotic expression vector pET-28a, the fusion proteins HSP and SAA are subjected to fusion expression, SAA expression is efficient, the expression level of SAA accounts for 25% of the total protein of whole bacteria, the expression form is soluble protein, the purpose of preparing a large number of SAA proteins for diagnosis is achieved, the HSP/SAA recombinant protein is good in stability, and the application prospect is wide. The application and preparation of the SAA in various diagnostic reagents are facilitated. 本发明公开了一种HSP/SAA重组蛋白及其制备方法和应用。所述HSP/SAA重组蛋白是由HSP基因与SAA基因通过BglII/BamHI同尾酶连接而成的。本发明将融合蛋白HSP以及SAA进行基因优化后，将其插入原核表达载体pET-28a中，使两者融合表达，并且SAA表达高效，其表达水平占全菌总蛋白的25％，且表达形式为可溶性蛋白，达到了大量制备