Primer pair, kit and detection method for detecting genotype of gold and silver feather sites of chicken
The invention discloses a primer pair, a kit and a detection method for detecting genotypes of gold and silver feather sites of chickens, and belongs to the technical field of animal genetic breeding and biology. A primer pair is designed aiming at the SLC45A2 gene mutation causing the silver feathe...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a primer pair, a kit and a detection method for detecting genotypes of gold and silver feather sites of chickens, and belongs to the technical field of animal genetic breeding and biology. A primer pair is designed aiming at the SLC45A2 gene mutation causing the silver feather phenotype, a mismatched base is introduced into a downstream primer, and the mismatched base is combined with the mutation of the gene to form a BspHI restriction enzyme cutting site, so that the primer can be used for PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) analysis. The genotype of the chicken gold and silver feather site (namely the SLC45A2 gene mutation site) can be judged according to an electrophoresis result by taking the genomic DNA of the chicken of which the genotype is to be judged as a template and carrying out PCR amplification by using the primer pair, and then carrying out enzyme digestion and agarose gel electrophoresis detection on a PCR product. Compared wit |
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