Nitric oxide light-activated fluorescent probe as well as preparation method and application thereof
The invention relates to a nitric oxide light-activated fluorescent probe and a preparation method and application thereof, the structural formula of the probe is shown in the specification, o-nitrobenzyl is selected as a photosensitive group, photolysis reaction is rapid, and the nitric oxide light...
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| creator | ZHANG LING YING WEIWU LI XUEJIAO ZHENG YOUGUANG WU CHUNLI XUE YUNSHENG |
| description | The invention relates to a nitric oxide light-activated fluorescent probe and a preparation method and application thereof, the structural formula of the probe is shown in the specification, o-nitrobenzyl is selected as a photosensitive group, photolysis reaction is rapid, and the nitric oxide light-activated fluorescent probe has the advantages of low detection limit (13nM), high signal-to-noise ratio, high fluorescence response multiple (about 500 times), high sensitivity, good selectivity and the like. The fluorescence intensity and the NO (0-20 mu M) concentration have a good linear relationship, which indicates that the probe is suitable for quantitative detection of nitric oxide in a living body; and PNO is further applied to realize fluorescence imaging of nitric oxide of RAW 264.7 cells.
本发明涉及一种一氧化氮光激活荧光探针及其在制备方法和应用，该探针的结构式如下所示，选用邻硝基苄基作为光敏基团，光解反应迅速，具有检测限低(13nM)，信噪比高，荧光响应倍数高(约500倍)，灵敏度高，选择性好等优势。荧光强度与NO(0-20μM)浓度之间具有良好的线性关系，说明探针适合于生物体内一氧化氮的定量检测；并进一步应用PNO实现了RAW 264.7细胞一氧化氮的荧光成像。 |
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本发明涉及一种一氧化氮光激活荧光探针及其在制备方法和应用，该探针的结构式如下所示，选用邻硝基苄基作为光敏基团，光解反应迅速，具有检测限低(13nM)，信噪比高，荧光响应倍数高(约500倍)，灵敏度高，选择性好等优势。荧光强度与NO(0-20μM)浓度之间具有良好的线性关系，说明探针适合于生物体内一氧化氮的定量检测；并进一步应用PNO实现了RAW 264.7细胞一氧化氮的荧光成像。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | ADHESIVES CHEMISTRY DYES HETEROCYCLIC COMPOUNDS INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE MEASURING METALLURGY MISCELLANEOUS APPLICATIONS OF MATERIALS MISCELLANEOUS COMPOSITIONS NATURAL RESINS ORGANIC CHEMISTRY PAINTS PHYSICS POLISHES TESTING |
| title | Nitric oxide light-activated fluorescent probe as well as preparation method and application thereof |
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