Simple and convenient high-throughput DNA methylation detection method

The invention provides a simple and convenient high-throughput DNA methylation detection method, which comprises the following steps: (1) carrying out oxidation treatment on a DNA sample by using strong base to oxidize cytosine in the DNA sample into uracil and oxidize methylated cytosine into thymine; (2) adding acid to neutralize strong base, and controlling the pH value of the reaction system within 6-8; and (3) carrying out library amplification and sequencing by adopting uracil-intolerant high-fidelity DNA polymerase. The invention develops a simple and convenient high-throughput DNA cytosine methylation detection technology which is named as DMA-seq. Compared with an existing DNA methylation detection kit, the DMA-seq has the advantages of being easy to operate, short in consumed time, small in DNA damage, good in data quality, high in methylation site targeting performance and the like, and is suitable for high-abundance DNA methylation site detection, especially in the early tumor screening direction.