Application of braA08g039212E gene derived from European turnip ECD04 in plasmodiophora brassicae resistance improvement

The invention discloses an application of a BraA08g039212E gene derived from European turnip ECD04 in plasmodiophora brassicae resistance improvement, and discloses a resistance effect of the BraA08g039212E gene derived from European turnip ECD04 on a Chinese plasmodiophora brassicae No.4 physiologi...

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Bibliographische Detailangaben
Hauptverfasser:	ZHANG CHUNYU, SHEN JICHENG, GAN LONGCAI, DUN BICHENG, CHEN PENG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM ORGANIC CHEMISTRY PEPTIDES PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses an application of a BraA08g039212E gene derived from European turnip ECD04 in plasmodiophora brassicae resistance improvement, and discloses a resistance effect of the BraA08g039212E gene derived from European turnip ECD04 on a Chinese plasmodiophora brassicae No.4 physiological race, a nucleotide sequence of the gene is shown as SEQ ID No.1 or SEQ ID No.2, and an amino acid sequence of the gene is shown as SEQ ID No.3. The method comprises the following steps: finely positioning the gene by utilizing a map-based cloning method; the physical position of the gene on an A08 chromosome is determined, and then the gDNA or cDNA of the gene is used for cloning and genetic transformation of rape which is not resistant to clubroot, so that the clubroot resistance function of the gene on plasmodiophora brassicae is verified. Scientific and technical bases can be provided for breeding new varieties of brassica anti-clubroot plants through a genetic engineering means or a molecular marker-assiste