Application of chromatographic filler taking Oligo (dT) as affinity ligand
The invention discloses an application of a chromatographic filler taking Oligo (dT) as an affinity ligand, and discloses an application of the chromatographic filler in an mRNA (messenger Ribonucleic Acid) purification process, and the purification process comprises the following steps: a, preparin...
Gespeichert in:
| Hauptverfasser: | , , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | HU XINMEI LYU XIAOLIN DING LIANGLONG MAO HUIMING QI TIANMING |
| description | The invention discloses an application of a chromatographic filler taking Oligo (dT) as an affinity ligand, and discloses an application of the chromatographic filler in an mRNA (messenger Ribonucleic Acid) purification process, and the purification process comprises the following steps: a, preparing a sample; b, loading a sample; c, washing impurities; d, eluting; and e, in-place cleaning. According to the purification method adopting the filler, effective capture, impurity removal and elution of mRNA can be realized, subsequent purification steps are simplified, the overall production efficiency of downstream purification is improved to the maximum extent, and the purification method is suitable for industrial large-scale production. In addition, if a Poly (A) structure exists in a circular RNA sequence, the column chromatography is also applicable.
本发明公开以Oligo(dT)为亲和配基的层析填料的用途，公开该层析填料在纯化mRNA工艺中的用途，纯化的工艺包括：a.制样；b.上样；c.洗杂；d.洗脱；e.在位清洗。本发明采用上述填料的纯化方法能实现mRNA的有效捕获、除杂和洗脱，简化后续纯化步骤并最大限度地提高下游纯化整体生产效率，适合工业放大生产。此外，环形R |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN114381454A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN114381454A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN114381454A3</originalsourceid><addsrcrecordid>eNqNyrEKwjAQgOEsDqK-w7nVwSE0gmspFXHQpXs50kt6GHMhyeLb6-ADOP3w8a_VrUspsMXKEkEc2CXLC6v4jGlhC45DoAwVnxw9PAJ7gWYeD4AF0DmOXN_wVYzzVq0chkK7XzdqfxnG_nqkJBOVhJYi1am_a23aszYn07X_PB_nYTRv</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Application of chromatographic filler taking Oligo (dT) as affinity ligand</title><source>esp@cenet</source><creator>HU XINMEI ; LYU XIAOLIN ; DING LIANGLONG ; MAO HUIMING ; QI TIANMING</creator><creatorcontrib>HU XINMEI ; LYU XIAOLIN ; DING LIANGLONG ; MAO HUIMING ; QI TIANMING</creatorcontrib><description>The invention discloses an application of a chromatographic filler taking Oligo (dT) as an affinity ligand, and discloses an application of the chromatographic filler in an mRNA (messenger Ribonucleic Acid) purification process, and the purification process comprises the following steps: a, preparing a sample; b, loading a sample; c, washing impurities; d, eluting; and e, in-place cleaning. According to the purification method adopting the filler, effective capture, impurity removal and elution of mRNA can be realized, subsequent purification steps are simplified, the overall production efficiency of downstream purification is improved to the maximum extent, and the purification method is suitable for industrial large-scale production. In addition, if a Poly (A) structure exists in a circular RNA sequence, the column chromatography is also applicable.
本发明公开以Oligo(dT)为亲和配基的层析填料的用途，公开该层析填料在纯化mRNA工艺中的用途，纯化的工艺包括：a.制样；b.上样；c.洗杂；d.洗脱；e.在位清洗。本发明采用上述填料的纯化方法能实现mRNA的有效捕获、除杂和洗脱，简化后续纯化步骤并最大限度地提高下游纯化整体生产效率，适合工业放大生产。此外，环形R</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PERFORMING OPERATIONS ; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SEPARATION ; SPIRITS ; TRANSPORTING ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220422&DB=EPODOC&CC=CN&NR=114381454A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220422&DB=EPODOC&CC=CN&NR=114381454A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HU XINMEI</creatorcontrib><creatorcontrib>LYU XIAOLIN</creatorcontrib><creatorcontrib>DING LIANGLONG</creatorcontrib><creatorcontrib>MAO HUIMING</creatorcontrib><creatorcontrib>QI TIANMING</creatorcontrib><title>Application of chromatographic filler taking Oligo (dT) as affinity ligand</title><description>The invention discloses an application of a chromatographic filler taking Oligo (dT) as an affinity ligand, and discloses an application of the chromatographic filler in an mRNA (messenger Ribonucleic Acid) purification process, and the purification process comprises the following steps: a, preparing a sample; b, loading a sample; c, washing impurities; d, eluting; and e, in-place cleaning. According to the purification method adopting the filler, effective capture, impurity removal and elution of mRNA can be realized, subsequent purification steps are simplified, the overall production efficiency of downstream purification is improved to the maximum extent, and the purification method is suitable for industrial large-scale production. In addition, if a Poly (A) structure exists in a circular RNA sequence, the column chromatography is also applicable.
本发明公开以Oligo(dT)为亲和配基的层析填料的用途，公开该层析填料在纯化mRNA工艺中的用途，纯化的工艺包括：a.制样；b.上样；c.洗杂；d.洗脱；e.在位清洗。本发明采用上述填料的纯化方法能实现mRNA的有效捕获、除杂和洗脱，简化后续纯化步骤并最大限度地提高下游纯化整体生产效率，适合工业放大生产。此外，环形R</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PERFORMING OPERATIONS</subject><subject>PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SEPARATION</subject><subject>SPIRITS</subject><subject>TRANSPORTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyrEKwjAQgOEsDqK-w7nVwSE0gmspFXHQpXs50kt6GHMhyeLb6-ADOP3w8a_VrUspsMXKEkEc2CXLC6v4jGlhC45DoAwVnxw9PAJ7gWYeD4AF0DmOXN_wVYzzVq0chkK7XzdqfxnG_nqkJBOVhJYi1am_a23aszYn07X_PB_nYTRv</recordid><startdate>20220422</startdate><enddate>20220422</enddate><creator>HU XINMEI</creator><creator>LYU XIAOLIN</creator><creator>DING LIANGLONG</creator><creator>MAO HUIMING</creator><creator>QI TIANMING</creator><scope>EVB</scope></search><sort><creationdate>20220422</creationdate><title>Application of chromatographic filler taking Oligo (dT) as affinity ligand</title><author>HU XINMEI ; LYU XIAOLIN ; DING LIANGLONG ; MAO HUIMING ; QI TIANMING</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN114381454A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2022</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PERFORMING OPERATIONS</topic><topic>PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SEPARATION</topic><topic>SPIRITS</topic><topic>TRANSPORTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>HU XINMEI</creatorcontrib><creatorcontrib>LYU XIAOLIN</creatorcontrib><creatorcontrib>DING LIANGLONG</creatorcontrib><creatorcontrib>MAO HUIMING</creatorcontrib><creatorcontrib>QI TIANMING</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>HU XINMEI</au><au>LYU XIAOLIN</au><au>DING LIANGLONG</au><au>MAO HUIMING</au><au>QI TIANMING</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Application of chromatographic filler taking Oligo (dT) as affinity ligand</title><date>2022-04-22</date><risdate>2022</risdate><abstract>The invention discloses an application of a chromatographic filler taking Oligo (dT) as an affinity ligand, and discloses an application of the chromatographic filler in an mRNA (messenger Ribonucleic Acid) purification process, and the purification process comprises the following steps: a, preparing a sample; b, loading a sample; c, washing impurities; d, eluting; and e, in-place cleaning. According to the purification method adopting the filler, effective capture, impurity removal and elution of mRNA can be realized, subsequent purification steps are simplified, the overall production efficiency of downstream purification is improved to the maximum extent, and the purification method is suitable for industrial large-scale production. In addition, if a Poly (A) structure exists in a circular RNA sequence, the column chromatography is also applicable.
本发明公开以Oligo(dT)为亲和配基的层析填料的用途，公开该层析填料在纯化mRNA工艺中的用途，纯化的工艺包括：a.制样；b.上样；c.洗杂；d.洗脱；e.在位清洗。本发明采用上述填料的纯化方法能实现mRNA的有效捕获、除杂和洗脱，简化后续纯化步骤并最大限度地提高下游纯化整体生产效率，适合工业放大生产。此外，环形R</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN114381454A |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SEPARATION SPIRITS TRANSPORTING VINEGAR WINE |
| title | Application of chromatographic filler taking Oligo (dT) as affinity ligand |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T05%3A05%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=HU%20XINMEI&rft.date=2022-04-22&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN114381454A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |