Application of chromatographic filler taking Oligo (dT) as affinity ligand

The invention discloses an application of a chromatographic filler taking Oligo (dT) as an affinity ligand, and discloses an application of the chromatographic filler in an mRNA (messenger Ribonucleic Acid) purification process, and the purification process comprises the following steps: a, preparing a sample; b, loading a sample; c, washing impurities; d, eluting; and e, in-place cleaning. According to the purification method adopting the filler, effective capture, impurity removal and elution of mRNA can be realized, subsequent purification steps are simplified, the overall production efficiency of downstream purification is improved to the maximum extent, and the purification method is suitable for industrial large-scale production. In addition, if a Poly (A) structure exists in a circular RNA sequence, the column chromatography is also applicable. 本发明公开以Oligo(dT)为亲和配基的层析填料的用途，公开该层析填料在纯化mRNA工艺中的用途，纯化的工艺包括：a.制样；b.上样；c.洗杂；d.洗脱；e.在位清洗。本发明采用上述填料的纯化方法能实现mRNA的有效捕获、除杂和洗脱，简化后续纯化步骤并最大限度地提高下游纯化整体生产效率，适合工业放大生产。此外，环形R