Metarhizium anisopliae rejuvenation method

The invention belongs to the field of plant protection, and particularly provides a metarhizium anisopliae rejuvenation method, which specifically comprises the following steps: selecting larvae of spodoptera frugiperda with good growth condition, carrying out starvation treatment for 4-7 days, then selecting live larvae, preparing conidia of metarhizium anisopliae NG-Ma040 into a suspension liquid by using Tween-80 with the mass concentration of 0.01-0.1%, adjusting the concentration to 1-10 * 10 < 8 >/mL, and carrying out rejuvenation on the metarhizium anisopliae NG-Ma040 conidia to obtain the metarhizium anisopliae NG-Ma040 metarhizium anisopliae NG-Ma040 metarhizium anisopliae. And soaking the starved spodoptera frugiperda larvae in the spore suspension for 10-30 seconds, absorbing water on the surface by using filter paper, culturing in an incubator, and performing spore separation and purification after spodoptera frugiperda bombyx batryticatus appears. Compared with a live insect rejuvenation method a