Method for detecting active ingredients in loquat lung-heat-clearing drink preparation by utilizing HPLC (High Performance Liquid Chromatography)

The invention relates to the technical field of quality control of traditional Chinese medicines, in particular to a method for detecting active ingredients in a loquat lung-heat-clearing drink preparation by utilizing HPLC (High Performance Liquid Chromatography). In the method, the detection conditions of HPLC (High Performance Liquid Chromatography) are as follows: a chromatographic column adopts an octadecyl silane bonded silica gel column; acetonitrile is used as a mobile phase A, 0.005 mol/L of a 1-sodium heptanesulfonate solution (the pH is adjusted to be 2 by phosphoric acid) is used as a mobile phase B, and gradient elution is carried out. By means of the method, multiple active ingredients in the loquat lung-heat-clearing drink can be simultaneously detected at a time within 60 min, and the detection method has good precision, stability and repeatability. 本申请涉及中药质量控制技术领域，具体涉及一种利用HPLC检测枇杷清肺饮制剂中活性成分的方法。该方法中，HPLC的检测条件为：色谱柱采用十八烷基硅烷键合硅胶柱；以乙腈为流动相A，以0.005mol/L的1-庚烷磺酸钠溶液(磷酸调节pH＝2)为流动相B，进行梯度洗脱。通过此方法，能够在60min