Recombinant adenovirus with anti-tumor immune function as well as preparation method and application of recombinant adenovirus

The invention provides a recombinant adenovirus with an anti-tumor immune function, a preparation method and application, an expression fusion protein CD137L-PP4 gene is inserted into an adenovirus vector to construct the recombinant adenovirus with the anti-tumor immune function, and the fusion protein CD137L-PP4 comprises a CD137L protein and PP4 polypeptide with a T cell activation function. The recombinant adenovirus can be expressed in vivo and can induce generation of specific anti-tumor cell immunity, so that immunotherapy of tumors is realized. Therefore, the compound can be used for preparing a medicine for inducing an organism to generate specific cellular immune response aiming at CD137L and/or inducing the organism to generate a medicine for inhibiting tumor cell proliferation. 本发明提供了一种具有抗肿瘤免疫功能重组腺病毒、制备方法及应用，本发明将一种表达融合蛋白CD137L-PP4基因插入腺病毒载体，构建获得具有抗肿瘤免疫功能重组腺病毒，所述融合蛋白CD137L-PP4包含CD137L蛋白和具有T细胞激活功能的PP4多肽。该重组腺病毒能在体内表达，可以诱导产生特异性的抗肿瘤细胞免疫，实现肿瘤的免疫治疗。因而可以用于制备用于诱导机体产生针对CD137L的特异性细胞免疫反应的药物和/或诱导机体产生抑制肿瘤细胞增殖的药物