Double-enzyme active cobalt monatomic nano-enzyme as well as preparation method and application thereof

The invention relates to a double-enzyme active cobalt monatomic nano-enzyme as well as a preparation method and application thereof. The double-enzyme active cobalt monatomic nano-enzyme comprises a carbon skeleton and Co monatomic anchored on the carbon skeleton, the double-enzyme active cobalt monatomic nano-enzyme is prepared by a method comprising the following steps: (1) Zn (NO3) 2.6 H2O, Co (NO3) 2.6 H2O and 2-methylimidazole are subjected to a reaction to synthesize Co-ZIF-8; and (2) carrying out pyrolysis on the Co-ZIF-8, and treating with H2SO4 to obtain the Co-ZIF-8. The Co-N/C SACs catalyst is of a monatomic structure, has double-enzyme activity and can simulate the activity of ascorbic acid oxidase and glutathione oxidase at the same time. 本发明涉及一种双酶活性钴单原子纳米酶及其制备方法、应用，该双酶活性钴单原子纳米酶包括碳骨架和锚定在碳骨架上的Co单原子；该双酶活性钴单原子纳米酶由包括如下步骤的方法制备得到：(1)Zn(NO3)2·6H2O、Co(NO3)2·6H2O和2-甲基咪唑反应合成Co-ZIF-8；(2)将Co-ZIF-8的热解和H2SO4处理后，制得。本发明Co-N/C SACs催化剂为单原子结构，具有双酶活性，可同时模拟抗坏血酸氧化酶和谷胱甘肽氧化酶的活性。