Tobacco endogenous anti-tobacco mosaic virus extract as well as preparation method and application thereof

The invention discloses a tobacco endogenous anti-tobacco mosaic virus extract as well as a preparation method and application thereof. The preparation method comprises the following steps: step 1, extraction: taking aired tobacco rhizomes, crushing, digesting for multiple times, and distilling to obtain an extract; step 2, purification: the obtained extract is sequentially subjected to concentration, chromatographic column loading, elution and distillation, and a concentrated solution is obtained; and step 3, drying: drying the concentrated solution, crushing, sieving with a 100-mesh sieve, and then collecting and sub-packaging. The large-scale production process and the quality control method of the endogenous antiviral components of the tobacco are formed by adopting the tobacco field wastes through solvent extraction and chromatographic separation methods, and the use method and the use range of the endogenous antiviral components of the tobacco are definite, so that a material basis and a scientific basi