Rapid identification method for polymorphism EST-SSRs of Yangtze River coilia ectenes based on transcriptome
The invention relates to the technical field of development of molecular markers for breeding Yangtze River coilia ectenes, in particular to a transcriptome-based rapid identification method for polymorphism EST-SSRs of Yangtze River coilia ectenes. On the basis of brain transcriptome data of bred Y...
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| creator | SHI YONGHAI ZHANG ZHIWEN YU AIQING LIU YONGSHI YAN YINLONG XU JIABO |
| description | The invention relates to the technical field of development of molecular markers for breeding Yangtze River coilia ectenes, in particular to a transcriptome-based rapid identification method for polymorphism EST-SSRs of Yangtze River coilia ectenes. On the basis of brain transcriptome data of bred Yangtze River coilia ectenes female and male individuals, 1082 polymorphic EST-SSRs (19.07%) are screened from 5675 bred Yangtze River coilia ectenes female and male transcriptome common EST-SSR sequences, 20 sites are randomly selected to design specific PCR amplification primers for validity and polymorphism verification, results show that 19 pairs of EST-SSRs can be amplified to obtain stable and clear bands, and 19 pairs of EST-SSRs can be amplified to obtain stable and clear EST-SSRs; wherein 18 pairs have polymorphism (the polymorphism rate is about 90%), the detection efficiency is high, and the current germplasm resource situation of the Yangtze River coilia ectenes cultured groups in different years can be |
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On the basis of brain transcriptome data of bred Yangtze River coilia ectenes female and male individuals, 1082 polymorphic EST-SSRs (19.07%) are screened from 5675 bred Yangtze River coilia ectenes female and male transcriptome common EST-SSR sequences, 20 sites are randomly selected to design specific PCR amplification primers for validity and polymorphism verification, results show that 19 pairs of EST-SSRs can be amplified to obtain stable and clear bands, and 19 pairs of EST-SSRs can be amplified to obtain stable and clear EST-SSRs; wherein 18 pairs have polymorphism (the polymorphism rate is about 90%), the detection efficiency is high, and the current germplasm resource situation of the Yangtze River coilia ectenes cultured groups in different years can be</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PHYSICS ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220415&DB=EPODOC&CC=CN&NR=114350814A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220415&DB=EPODOC&CC=CN&NR=114350814A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SHI YONGHAI</creatorcontrib><creatorcontrib>ZHANG ZHIWEN</creatorcontrib><creatorcontrib>YU AIQING</creatorcontrib><creatorcontrib>LIU YONGSHI</creatorcontrib><creatorcontrib>YAN YINLONG</creatorcontrib><creatorcontrib>XU JIABO</creatorcontrib><title>Rapid identification method for polymorphism EST-SSRs of Yangtze River coilia ectenes based on transcriptome</title><description>The invention relates to the technical field of development of molecular markers for breeding Yangtze River coilia ectenes, in particular to a transcriptome-based rapid identification method for polymorphism EST-SSRs of Yangtze River coilia ectenes. 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On the basis of brain transcriptome data of bred Yangtze River coilia ectenes female and male individuals, 1082 polymorphic EST-SSRs (19.07%) are screened from 5675 bred Yangtze River coilia ectenes female and male transcriptome common EST-SSR sequences, 20 sites are randomly selected to design specific PCR amplification primers for validity and polymorphism verification, results show that 19 pairs of EST-SSRs can be amplified to obtain stable and clear bands, and 19 pairs of EST-SSRs can be amplified to obtain stable and clear EST-SSRs; wherein 18 pairs have polymorphism (the polymorphism rate is about 90%), the detection efficiency is high, and the current germplasm resource situation of the Yangtze River coilia ectenes cultured groups in different years can be</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Rapid identification method for polymorphism EST-SSRs of Yangtze River coilia ectenes based on transcriptome |
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