Production and purification method and application of disulfide bond-containing polypeptide human brain natriuretic peptide hBNP

The invention relates to a production and purification method of disulfide bond-containing polypeptide human brain natriuretic peptide rhBNP. The method comprises the following steps: sequentially connecting a gene sequence of an aggregation peptide, a gene sequence of a cleavage tag and a gene sequence of human brain natriuretic peptide rhBNP to form a gene of a fusion protein, and introducing the gene of the fusion protein into a host cell to obtain an engineering bacterium; culturing engineering bacteria to express the fusion protein, then splitting the engineering bacteria, and centrifuging to take precipitate, so as to obtain an aggregate of the fusion protein; and cutting the aggregate of the fusion protein to obtain the human brain natriuretic peptide rhBNP. The yield and purity of the human brain natriuretic peptide rhBNP obtained through purification are very high, and especially after self-cutting and centrifugal operation, the purity of 99% or above can be achieved only through two-step standardize